Session 1:
Introduction to Hydrodynamic Theory and Analytical Ultracentrifugation

Session 2:
Sedimentation Velocity Analysis, Part I

Session 3:
Sedimentation Velocity Analysis, Part II - Interacting Models and Software Practice Sessions

Session 4:
Equilibrium Data Analysis and Wavelength Data Analysis

Session 5 - Bead Modeling (SOMO)

Sessions 6-9:
Session 6: Presentation of Data Analysis Results

Presentation of Data Analysis Results

Data Reports:

• Overlaid Distributions
• 3D distributions and Pseudo-3D distributions
• van Holde - Weischet Distributions
• Generating publication quality graphs

Coffee Break

Session 7: Mini Symposium on Advances in Analytical Ultracentrifugation

Topics: New and notable in Analytical Ultracentrifugation - what comes next?

Symposium Speakers and Talks:

• Dr. Mattia Rocco, IST, Genova, Italy - US-SOMO (SOlution MOdeler): overview, basic principles, and an example application to integrin conformation
  Abstract: In this presentation, I will first review the basic principles of macromolecular hydrodynamics as implemented in bead modeling approaches. The bead modeling methods available in the SOlution MOdeller suite within UltraScan (US-SOMO) will be then outlined, and their accuracy illustrated. An example application to the integrin solution conformation will be then briefly illustrated. Experimental sedimentation velocity and dynamic light scattering data on solubilized integrins, high molecular weight, modular, glycosylated transmembrane cellular receptors composed by two subunits, are compared with US-SOMO-computed parameters for atomic models based on crystallographic and NMR data. Furthermore, distance distribution functions for the models are also compared with the one derived from experimental small-angle neutron scattering (SANS) data. The results suggest that, contrary to current accepted models, resting integrins are already extended, and their activation is not necessarily linked to full tail separation.

• Dr. Helmut Cölfen, University of Konstanz, Germany - A Multiwavelength UV/Vis detector for the Analytical Ultracentrifuge
  Abstract: A multiwavelength UV/Vis detector was developed for the Analytical Ultracentrifuge, which adds the wavelength dimension to the up to now possible radial position and concentration readings. This detector is particularly useful for all systems, which change their light absorption properties with composition or size. The hardware setup and basic performance of this detector will be described. For the example of CdTe nanoparticles, the amount of information which is available from a single sedimentation velocity experiment will be demonstrated. CdTe nanoparticles < 10 nm show a blue shift in their absorption spectrum with decreasing particle size due to the quantum size effect. Using Ultrascan in a version extended for the multiwavelength detector, and applying 2-dimensional spectrum analysis, it was possible to determine the distributions of sedimentation coefficient, particle size, molar mass as well as nanoparticle shape of 24 nanoparticles in a mixture. The hydrodynamic particle size distribution is very high and in the Angström range whereas the spectra even resolve average particle sizes with atomar resolution. Such high resolution data allow conclusions about the nanoparticle growth mechanism. This example shows the wealth of information which can be obtained from a simple sedimentation velocity experiment on complex mixtures with sophisticated analysis using Ultrascan.

• Dr. Thomas Laue, University of New Hamshire - The role of AUC in post-reductionist Biochemistry
  Abstract: In order to be relevant to biology, biochemistry must move beyond its reductionist roots. Since biological systems tend to have very high concentrations of complex mixtures of molecules, the biochemistry and biophysics of these systems need to be understood. AUC is one of a very few techniques that can characterize such systems. For AUC to be useful in this regard, however, new instrumentation and new analysis methods need to be developed. This talk will focus on what we have learned so far from AUC, and what directions we need to pursue.

• Glen Ramsay, Aviv Biomedical - The Analytical Ultracentrifuge Fluorescence Detection System
  Abstract: In 2003 Aviv Biomedical Inc. licensed from the University of New Hampshire the design for an analytical ultracentrifuge fluorescence detection system (AU-FDS). It is capable of operating in both the Beckman - Coulter Inc. XL-A and XL-I analytical ultracentrifuges. The system is based on a confocal microscope design that uses a 488 nm laser as the light source. Fluorescent light in the range of 505 - 585 nm is measured. This talk will give an overview of the detector's operation and applications.

Special Topics

Open Discussion

• Feature Requests
• Participant Comments and Questions

Parallel Session 8:
Session 8: Topics in data analysis and software practice (in parallel with session 9)

Software Practice Session

Individual Tutoring Sessions

• Analysis of Experimental Data provided by participants
• Software Practice Sessions

Adjourn

Parallel Session 9:
Session 9: Instrument Workshop (in parallel with session 8)